Collin

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Towards middle-up evaluation of polyclonal antibodies: subclass-specific

Towards middle-up evaluation of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by the use of HPLC-MS   In latest years, superior HPLC-MS methods based mostly on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) evaluation have been carried out for the characterization of therapeutic monoclonal antibodies. Here, we assess feasibility of middle-up/middle-down evaluation for polyclonal …

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prokaryotic expression, polyclonal antibody preparation, and subcellular localization

The prokaryotic expression, polyclonal antibody preparation, and subcellular localization of the transmembrane protein NS2A of the duck Tembusu virus   Duck Tembusu virus (DTMUV) is a single-stranded, positive-sense RNA arbovirus, belonging to the genus Flavivirus, household Flaviviridae. As a transmembrane protein, non-structural protein 2A (NS2A) performs an essential position in virion meeting, replication complicated and antagonizing host immune …

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Preparation and Characterization of Two Immunogens and Production of Polyclonal Antibody

Preparation and Characterization of Two Immunogens and Production of Polyclonal Antibody with High Affinity and Specificity for Darunavir Darunavir (DRV) is a potent antiviral drug used for therapy of infections with human immunodeficiency virus (HIV). Effective and secure therapy with DRV requires its therapeutic drug monitoring (TDM) in affected person’s plasma throughout remedy. To assist TDM of DRV, …

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Development of a Highly Sensitive Enzyme-Linked Immunosorbent Assay

Development of a Highly Sensitive Enzyme-Linked Immunosorbent Assay for Mouse Soluble Epoxide Hydrolase Detection by Combining a Polyclonal Capture Antibody with a Nanobody Tracer   Enzyme-linked immunosorbent assays (ELISA) for the detection of soluble epoxide hydrolase (sEH), a key enzyme within the metabolism of fatty acids and a biomarker, could more and more characterize an essential diagnostic device. However, …

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Generation of High Affinity Anti-Peptide Polyclonal Antibodies

Generation of High Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat α s1-Casein   The chemical, technological and allergy properties of goat’s milk are considerably affected by the extent of αs1-casein. Detection and quantification of αs1-casein requires high-specificity strategies to beat high-sequence similarity between this protein and others within the casein household. Unavailability of antibodies with excessive affinity and specificity in …

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Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain

Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant CTLA-4 Protein.   Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is a critical negative immunomodulatory receptor that is normally expressed in activated T cells and noticeably, in many cancerous cells. Indeed, molecular detection of CTLA-4 protein is crucial in basic research. In this work, the extracellular domain of …

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Prokaryotic expression of mouse Stra8 and preparation and identification

Prokaryotic expression of mouse Stra8 and preparation and identification of rabbit anti-mouse Stra8 polyclonal antibody Objective To prokaryotically express mouse stimulated by retinoic acid gene 8 (Stra8) and prepare rabbit anti-mouse Stra8 polyclonal antibody. Methods The recombinant plasmid pET28a-Stra8 was constructed by cloning technology and identified by double enzyme digestion and sequencing, and then transformed into E. …

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Efficient detection of eukaryotic calcium-sensing receptor

Efficient detection of eukaryotic calcium-sensing receptor (CaSR) by polyclonal antibody against prokaryotic expressed truncated CaSR Calcium-sensing receptor (CaSR), which is better known for its action as regulating calcium homeostasis, can bind various ligands. To facilitate research on CaSR and understand the receptor’s function further, an in silico designed truncated protein was developed. The resulting protein folding indicated that …

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The pharyngeal nervous system orchestrates feeding behavior in planarians.

The pharyngeal nervous system orchestrates feeding behavior in planarians.

Planarians exhibit traits of cephalization however are distinctive amongst bilaterians in that they ingest meals by the use of goal-directed actions of a trunk-positioned pharynx, following protrusion of the pharynx out of the physique, elevating the query of how planarians management such a posh set of physique actions for attaining strong feeding. Here, we use …

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A comprehensive annotation and differential expression analysis of short and long non-coding RNAs in 16 bat genomes.

A comprehensive annotation and differential expression analysis of short and long non-coding RNAs in 16 bat genomes.

Although bats are more and more changing into the main focus of scientific research on account of their distinctive properties, these distinctive animals are nonetheless among the many least studied mammals. Assembly high quality and completeness of bat genomes differ loads and particularly non-coding RNA (ncRNA) annotations are incomplete or just lacking. Accordingly, customary bioinformatics pipelines for …

A comprehensive annotation and differential expression analysis of short and long non-coding RNAs in 16 bat genomes. Read More »