Hot air stream reduces cytotoxicity of light-cured calcium hydroxide based cements

Hot air stream reduces cytotoxicity of light-cured calcium hydroxide based cements

The light-cured calcium hydroxide based cements have incomplete polymerization and unconverted monomers may cause pulp cell harm. The intention of this research was to guage the affect of a heat and scorching air stream on the cytotoxicity of light-cured calcium hydroxide based cements.

The supplies Dycal (standard cement), Biocal, Hidrox-Cal, and Ultra-Blend Plus (light-cured calcium hydroxide cements) had been submitted to cytotoxicity evaluation after polymerization, with out vs. with earlier warmth therapy with a heat (37°C) and a scorching (60°C) air stream. Following polymerization, cements had been maintained in tradition medium for 24 hours and seven days, and subjected to the MTT check.

Data had been analyzed utilizing evaluation of variance (ANOVA) adopted by post-hoc Student-Newman-Keuls (<0.05).The outcomes indicated important variations between the supplies in response to their composition, i.e., light-cured cements handled with a jet of heat air confirmed comparable cytotoxicity ranges to these noticed for standard cement, suggesting that they could be thought of options in circumstances requiring pulp-capping therapy.

The light-cured calcium hydroxide based cements have incomplete polymerization and unconverted monomers may cause pulp cell harm. The intention of this research was to guage the affect of a heat and scorching air stream on the cytotoxicity of light-cured calcium hydroxide based cements.

Hot air stream reduces cytotoxicity of light-cured calcium hydroxide based cements
Hot air stream reduces cytotoxicity of light-cured calcium hydroxide based cements

The supplies Dycal (standard cement), Biocal, Hidrox-Cal, and Ultra-Blend Plus (light-cured calcium hydroxide cements) had been submitted to cytotoxicity evaluation after polymerization, with out vs. with earlier warmth therapy with a heat (37°C) and a scorching (60°C) air stream. Following polymerization, cements had been maintained in tradition medium for 24 hours and seven days, and subjected to the MTT check.

Data had been analyzed utilizing evaluation of variance (ANOVA) adopted by post-hoc Student-Newman-Keuls (<0.05).The outcomes indicated important variations between the supplies in response to their composition, i.e., light-cured cements handled with a jet of heat air confirmed comparable cytotoxicity ranges to these noticed for standard cement, suggesting that they could be thought of options in circumstances requiring pulp-capping therapy.

Data had been analyzed utilizing evaluation of variance

First launched in Germany in 1920, calcium hydroxide has been the biomaterial most extensively studied through the years, with favorable medical outcomes (1). Calcium hydroxide could also be used immediately on pulp tissues or indi-rectly as pulp-capping materials (2). It has a protracted historical past of medical success and continues to be the gold commonplace when the purpose is to guard the dentin-pulp advanced (3,4).

The primary benefit of calcium hydroxide is its alkaline pH, because it creates an optimum setting for the sti-mulation of alkaline phosphatase, important to the forma-tion of mineralized barrier (5). From a unique standpoint, calcium hydroxide presents a low modulus of elasticity and low resistance to com-pression, which prohibit its use in skinny layers, in particular areas (6).

The restricted mechanical properties of chemi-cally activated calcium hydroxide, mixed with the requirement to accommodate the fabric in areas with the eminence of pulp publicity, has resulted within the improvement of light-curable calcium hydroxide based cements, composed of methacrylate monomers, with the next benefits: elevated resistance, low solubi-lity in acid settings, managed working time, and achie-vement of optimum mechanical properties instantly after the light-curing course of (7).

However, when resin supplies are utilized to deep cavi-ties, diffusion of unpolymerized monomers into pulp tis-sues could happen via the dentinal tubules (8-10) and trigger poisonous results (11-13). This state of affairs can grow to be extra extreme when the monomers come into direct con-tact with the dental pulp (14).

Therefore, enough con-version of monomers into polymers is important to maxi-mize the bodily properties and medical efficiency of light-curable calcium hydroxide based cements, in addition to to cut back their cytotoxicity (15). Among a number of exis ting methods to boost polymerization charges, the use of excessive temperatures both earlier than or throughout polymerization stands out (16-18). Several authors have demonstrated hi-gher polymerization charges and due to this fact superior bodily (16,19) and organic (20) properties of resin supplies after the use of light-curing strategies using warmth.A elementary characteristic for these supplies is biocompati-bility (21) and step one in assessing biocompatibility is thru cytotoxicity evaluation based on mobile conduct and viability (22).

Methacrylate-based dental supplies are recognized to current a excessive degree of cytotoxicity, and are due to this fact more likely to penetrate the pulp and induce cytotoxic results (23). Therefore, the intention of this research was to eva-luate the affect of a heat and scorching air stream on the cytotoxicity of light-cured calcium hydroxide cements. Material and Methods-Sample preparation

A traditional cement – Dycal (Dentsply – Milford, DE, USA) and three light-cured calcium hydroxide based cements: Biocal (Biodinâmica, Ibiporã, PR, Brazil), Hi-drox-Cal (Maquira, Maringá, PR, Brazil), Ultra-Blend Plus (Ultradent Products Inc., South Jordan, UT, USA) had been used on this in vitro research (Table 1).

Conventional cement was combined by 10s earlier than warmth therapy. All ce-ments had been handled in three completely different types: 1) no warmth therapy; 2) therapy with a jet of heat air (37°C); 3) therapy with a jet of scorching air (60ºC). The jet air appli-cation lasted 5 seconds, distant 10 cm from the slide. All light-curable specimens had been light-cured for 20 seconds utilizing a VALO cordless light-emitting diode (LED) cu-ring unit (Ultradent, Salt Lake City, UT, USA). The sam-ples measuring 9 mm diameter x 1 mm thickness had been ready and instantly sterilized with ethylene oxide

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