Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges.

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Towards middle-up evaluation of polyclonal antibodies: subclass-specific

Towards middle-up evaluation of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by the use of HPLC-MS   In latest years, superior HPLC-MS methods based mostly on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) evaluation have been carried out for the characterization of therapeutic monoclonal antibodies. Here, we assess feasibility of middle-up/middle-down evaluation for polyclonal …

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prokaryotic expression, polyclonal antibody preparation, and subcellular localization

The prokaryotic expression, polyclonal antibody preparation, and subcellular localization of the transmembrane protein NS2A of the duck Tembusu virus   Duck Tembusu virus (DTMUV) is a single-stranded, positive-sense RNA arbovirus, belonging to the genus Flavivirus, household Flaviviridae. As a transmembrane protein, non-structural protein 2A (NS2A) performs an essential position in virion meeting, replication complicated and antagonizing host immune …

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Preparation and Characterization of Two Immunogens and Production of Polyclonal Antibody

Preparation and Characterization of Two Immunogens and Production of Polyclonal Antibody with High Affinity and Specificity for Darunavir Darunavir (DRV) is a potent antiviral drug used for therapy of infections with human immunodeficiency virus (HIV). Effective and secure therapy with DRV requires its therapeutic drug monitoring (TDM) in affected person’s plasma throughout remedy. To assist TDM of DRV, …

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Development of a Highly Sensitive Enzyme-Linked Immunosorbent Assay

Development of a Highly Sensitive Enzyme-Linked Immunosorbent Assay for Mouse Soluble Epoxide Hydrolase Detection by Combining a Polyclonal Capture Antibody with a Nanobody Tracer   Enzyme-linked immunosorbent assays (ELISA) for the detection of soluble epoxide hydrolase (sEH), a key enzyme within the metabolism of fatty acids and a biomarker, could more and more characterize an essential diagnostic device. However, …

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Generation of High Affinity Anti-Peptide Polyclonal Antibodies

Generation of High Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat α s1-Casein   The chemical, technological and allergy properties of goat’s milk are considerably affected by the extent of αs1-casein. Detection and quantification of αs1-casein requires high-specificity strategies to beat high-sequence similarity between this protein and others within the casein household. Unavailability of antibodies with excessive affinity and specificity in …

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Prokaryotic expression of mouse Stra8 and preparation and identification

Prokaryotic expression of mouse Stra8 and preparation and identification of rabbit anti-mouse Stra8 polyclonal antibody Objective To prokaryotically express mouse stimulated by retinoic acid gene 8 (Stra8) and prepare rabbit anti-mouse Stra8 polyclonal antibody. Methods The recombinant plasmid pET28a-Stra8 was constructed by cloning technology and identified by double enzyme digestion and sequencing, and then transformed into E. …

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Efficient detection of eukaryotic calcium-sensing receptor

Efficient detection of eukaryotic calcium-sensing receptor (CaSR) by polyclonal antibody against prokaryotic expressed truncated CaSR Calcium-sensing receptor (CaSR), which is better known for its action as regulating calcium homeostasis, can bind various ligands. To facilitate research on CaSR and understand the receptor’s function further, an in silico designed truncated protein was developed. The resulting protein folding indicated that …

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Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges.

Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges.

Exploiting the RNA interference (RNAi) gene mechanism to silence important genes in pest bugs, main to poisonous results, has surfaced as a promising new management technique prior to now decade. While the primary business RNAi-based merchandise are presently coming to market, the applying towards a variety of insect species continues to be hindered by plenty of challenges. In this …

Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges. Read More »