Towards middle-up evaluation of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by the use of HPLC-MS In latest years, superior HPLC-MS methods based mostly on intact protein ("top-down") or protein subunit ("middle-up/middle-down") evaluation have been carried out for the characterization of therapeutic monoclonal antibodies. Here, we assess feasibility of middle-up/middle-down evaluation...
The prokaryotic expression, polyclonal antibody preparation, and subcellular localization of the transmembrane protein NS2A of the duck Tembusu virus Duck Tembusu virus (DTMUV) is a single-stranded, positive-sense RNA arbovirus, belonging to the genus Flavivirus, household Flaviviridae. As a transmembrane protein, non-structural protein 2A (NS2A) performs an essential position in virion meeting, replication complicated and antagonizing...
Preparation and Characterization of Two Immunogens and Production of Polyclonal Antibody with High Affinity and Specificity for Darunavir Darunavir (DRV) is a potent antiviral drug used for therapy of infections with human immunodeficiency virus (HIV). Effective and secure therapy with DRV requires its therapeutic drug monitoring (TDM) in affected person's plasma throughout remedy. To assist TDM of DRV, a selected antibody with excessive...
Generation of High Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat α s1-Casein The chemical, technological and allergy properties of goat's milk are considerably affected by the extent of αs1-casein. Detection and quantification of αs1-casein requires high-specificity strategies to beat high-sequence similarity between this protein and others within the casein household. Unavailability of antibodies with excessive affinity...
Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant CTLA-4 Protein. Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is a critical negative immunomodulatory receptor that is normally expressed in activated T cells and noticeably, in many cancerous cells. Indeed, molecular detection of CTLA-4 protein is crucial in basic research. In this work, the extracellular domain...
