Towards middle-up evaluation of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by the use of HPLC-MS In latest years, superior HPLC-MS methods based mostly on intact protein ("top-down") or protein subunit ("middle-up/middle-down") evaluation have been carried out for the characterization of therapeutic monoclonal antibodies. Here, we assess feasibility of middle-up/middle-down evaluation...
The prokaryotic expression, polyclonal antibody preparation, and subcellular localization of the transmembrane protein NS2A of the duck Tembusu virus Duck Tembusu virus (DTMUV) is a single-stranded, positive-sense RNA arbovirus, belonging to the genus Flavivirus, household Flaviviridae. As a transmembrane protein, non-structural protein 2A (NS2A) performs an essential position in virion meeting, replication complicated and antagonizing...
Development of a Highly Sensitive Enzyme-Linked Immunosorbent Assay for Mouse Soluble Epoxide Hydrolase Detection by Combining a Polyclonal Capture Antibody with a Nanobody Tracer Enzyme-linked immunosorbent assays (ELISA) for the detection of soluble epoxide hydrolase (sEH), a key enzyme within the metabolism of fatty acids and a biomarker, could more and more characterize an essential diagnostic device. However, there's a lack of ELISAs...
Generation of High Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat α s1-Casein The chemical, technological and allergy properties of goat's milk are considerably affected by the extent of αs1-casein. Detection and quantification of αs1-casein requires high-specificity strategies to beat high-sequence similarity between this protein and others within the casein household. Unavailability of antibodies with excessive affinity...
Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant CTLA-4 Protein. Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is a critical negative immunomodulatory receptor that is normally expressed in activated T cells and noticeably, in many cancerous cells. Indeed, molecular detection of CTLA-4 protein is crucial in basic research. In this work, the extracellular domain...
